All strains of newcastle disease virus will grow in the cells lining the allantoic cavity. Work in millers lab could one day lead to a universal flu vaccin. Good pasture in 1931 ad first used the embryonated hens egg. In the yolk sac, which is nothing else than the egg yolk, we can grow the herpes simplex virus. A hole is drilled in the shell of the embryonated egg, and a viral suspension or suspected virus containing tissue is injected into the fluid of the egg. Virus inoculation is carried out by injection of virus stock into the allantoic cavity using a needle.
Viruses which are not cultivated in embryonated egg and tissue culture are. At first egg is kept in incubator for embryo development up to 712 days and then virus sample is inoculated into the egg. This method is also important to grow the virus for other purposes such as. Cultivation of canine hepatitis virus in embryonated hens. Embryonated hens eggs are inoculated with animal viruses for the purpose of identification, isolation, titration and for quantity cultivation in the production of viruses. Influenza a viruses have significantly different host properties webster, 1997 and possibly different in vitro growth properties, depending on the strain. Embryonated egg culture accessscience from mcgrawhill. Opening in egg should be shield with paraffin and it is incubated for sufficient time. Generally three methods are employed for the virus cultivation.
Cultivation of viruses in embryonated egg culture answers. Influenza virus propagation in embryonated chicken eggs. Human influenza viruses can be amplified in a variety of mammalian cell substrates. The process of cultivating embryonated hens eggs inoculated with animal viruses for scientific purposes, including the production of viral vaccines. Viruses can be cultivated within suitable hosts, such as a living cell. Pdf influenza virus propagation in embryonated chicken eggs. Pdf the embryonated chicken egg ece provides a convenient, spacesaving incubator for the cultivation of many kinds of animal viruses where the egg.
Procedure for the isolation and detection of viruses from avian species using embryonated eggs this protocol is a copy of the standard operating procedure used by the avian influenza crl at the animal and plant health agency. Viruses are inoculated into chick embryo of 712 days old. Pdf infection and propagation of crimeancongo hemorrhagic. For virus cultivation, an egg embryo of 712 days is used. Good pasture in 1931 first used the embryonated hens egg for the cultivation of virus. Infectious disease researcher matthew miller shows how his lab grows the flu inside an egg.
Techniques of virus cultivation online microbiology notes. Convenient, inexpensive host for many animal species if any viral growth occurs, it will be signaled by death of embryo embryo cell damage. Atcc virology guide tips and techniques for propagating virus in tissue culture and embryonated chicken eggs. Cam chorioallantoic membrane, allantoic cavity, amniotic sac and yolk sac innoculation. Use of embryonated chicken eggs to grow the virus is the superior method since infectious stocks of all strains of ndv result. Embryonated eggs were inoculated with the virus and incubated for 48. The process of cultivation of viruses in embryonated eggs depends on the type of egg which is used. Pdf newcastle disease virus ndv strains have been divided into three.
However, generating high titer viruses in large quantities are best achieved in embryonated eggs. The virus cultivation in embryonated eggs involves three phases. Stocks of avirulent ndv prepared in tissue culture are noninfectious. Healthy embryos will have an air sac, welldeveloped blood vessels, and. Inoculation of embryonated eggs by the allantoic cavity route. Cultivation of canine hepatitis virus in embryonated hens eggs and its subsequent transmission to dogs. Comparison of embryonated chicken eggs with mdck cell. The opening is sealed with paraffin wax and the egg is incubated at 3738oc for the time required for the growth of virus. Additionally, all eggs should be candled to ensure viability and properly developed embryos. The egg used for cultivation must be sterile and the shell should be intact and healthy. Procedure for the isolation and detection of viruses from.